Revolutionizing Cell Metabolism Analysis
What is the Live Cell Metabolic Analyzer?
The live cell metabolic analyzer is a system used to observe and quantify the metabolic activity of living cells in real-time without disturbing their native environment. By a measurement of the primary metabolic parameters of glucose utilization and, lactate production, the analyzer provides one with insight into the response of cells under conditions over time. Unlike traditional metabolic assays, which are generally cell-extractive and stressful to cells, the technology allows for real-time observation of information, providing dynamic, high-resolution information that accurately reflects the real-time cellular state and long term trends.
How Does the Live Cell Metabolic Analyzer Work?
New sensors at the Live Cell Metabolic Analyzer center track even the smallest changes in the metabolic processes of viable cells. The sensors measure a series of significant parameters, though not restricted to the following:
- Glucose consumption
- Lactate production
- Metabolic rate of glycolysis pathway
It does this by measuring these parameters as cells convert nutrients to energy and by-products. Recording in real time allows researchers to make measurements of cell responses to stimulus, treatment, or environmental change without interfering with the cells themselves. As a non-interference, non-destructive technique for metabolic profiling, it has made it very valuable for research into cellular processes such as glycolysis and metabolism shifts.
Continuous, sampling-free measurement of glucose and lactate in culture medium. Visualize real-time changes in cell metabolism.
Cells are constantly growing and differentiating, and these processes are known to be closely linked to metabolism. In the field of cancer immunology, stem cell research, and the development of manufacturing processes for cell-based formulations, understanding the metabolic state of cells is a factor of ever-growing importance in the analysis of cell activation and disease. Continuous analysis of cell metabolism offers the ability to visualize the state of cells in real time, creating opportunities for unprecedented new discoveries in cell metabolism. The PHCbi Live cell metabolic analyzer will open new doors to those discoveries.
In-Line sensors: the key to continuous measurements of glucose and lactate
The glycolytic pathway is one of the main components of cellular energy metabolism. During glycolysis, glucose is taken up into cells and lactate is produced. Conventional analysis of cell metabolism typically involves estimating glucose and lactate concentrations from data points obtained from periodic sampling. With its unique high-precision In-Line sensors, the PHCbi Live cell metabolic analyzer offers these unique advantages:
- real-time monitoring of glucose and lactate concentrations
- continuous measurements
- no sampling of the culture medium and the same cells can also be used for separate evaluation after measurement
Measure cells in their usual culture environment
The simple design of the PHCbi Live cell metabolic analyzer makes it suitable for any laboratory space. Plus, there is no need for specialized cell culture equipment. Common commercial products (culture medium, 24-well plates, calibration liquid, additive reagents) can be used for cell culture. The Sensor module and Plate adaptor can be attached to a standard 24-well plate. Then, once the plate is placed in the Detector pre-installed within the CO2 incubator, real-time measurements can be checked easily using the touch-panel controller. Optional plate adapters for five different commercial 24-well plates are available.
Evaluate glycolytic changes directly
Changes in the glycolytic pathway can be evaluated directly by measuring the culture medium concentrations of glucose taken up by cells and lactate produced. With the PHCbi Live cell metabolic analyzer, the state of cell metabolism can be visualized as the rate of metabolic change using the consumption rate and production rate based on concentration values. Monitoring the efficiency of conversion from glucose to lactate makes it possible to evaluate not only glycolysis,
but also changes in the balance with other cellular metabolic processes, such as oxidative phosphorylation.
Customer Testimonial
Using PHCbi’s new LiCellMo now brings many benefits, such as provision of a detailed online readout of the glucose and lactate. The LiCellMo instrument is quite user friendly. It is very easy to handle. Particularly in the steps of preparation of the cell seeding onto the plate. Read the full story here.
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Key Attributes of the Live Cell Metabolic Analyzer
The Live Cell Metabolic Analyzer has the following attributes that qualify it to be a superior research and clinical study device:
Real-Time, long-term Monitoring: Provides real-time metabolic data for live cells up to 10 days, enabling scientists to observe dynamic change over time.
High Sensitivity: Picks up even subtle changes in metabolic activity, offering unprecedented resolution in metabolic measurement.
Reusability/ flexibility: cells can be reused/expanded further after LiCellMo measurement, since the cells are maintained in natural cell culture environment.
High Cell Compatibility: It is suitable for a variety of cell types, such as primary cells, immortalized cell lines, adherent cells spheroids, and some 3D cell cultures, and therefore can be used in various research applications.
Multisensor System: It uses a multisensor system to measure glucose, lactate, and metabolic rate simultaneously, providing an augmented perspective on cellular metabolism.
Both forms of data, with not only a knowledge of how large the metabolism is, but also cellular dynamics underlying it.
Applications of the Live Cell Metabolic Analyzer in Medicine and Research
Application of Live Cell Metabolic Analyzer to Cancer Research
Tumor cells also have modified metabolic profiles from normal cells with the reliance on glycolysis (the Warburg effect) and oxidative phosphorylation to generate energy. Live Cell Metabolic Analyzer is essential to the study of cancer because one can monitor very closely the metabolism of tumor cells and tumor microenvironment. It gives one a great opportunity to see how cancer cells modify their metabolism under various treatment or environmental stresses, thus giving important information in the planning of targeted therapies.
For instance, the analyzer can identify the switch from anaerobic to aerobic metabolism in tumors; this is considered to be a possible biomarker for early diagnosis or targets of precision (immuno-)oncology treatments.
Live Cell Metabolic Analyzer for Stem Cell Research
Research on stem cells is heavily based on what one knows as far as the mechanism that causes cells to differentiate and stay pluripotent. The metabolic change is very much part of the process. This will give a possible insight into the metabolic change with stem cells differentiation by tracking key stem cells metabolites. Scientists can gain insight as to possible roles which these processes may have to perform in stem cell formation and tissue regeneration, for example, glycolysis and metabolism swifts.
This ability to monitor stem cell viability and differentiation capacity in real time makes it extremely valuable in regenerative medicine, tissue engineering, and cell therapy studies.
Live Cell Metabolic Analyzer in Metabolic Diseases
It is worth learning the way cells metabolize energy and nutrients while studying metabolic disease so that effective treatment strategies can be created. The Live Cell Metabolic Analyzer is capable of delivering real-time information on lactate production, glucose consumption and glycolysis metabolism, that are all of great importance to the study of diabetes, obesity, liver, cardiovascular and mitochondrial diseases. From monitoring these metabolic processes, researchers are able to gain new insights into the processes of disease and drug targets for metabolic diseases.
Live Cell Metabolic Analyzer in Drug Discovery and Development
Live Cell Metabolic Analyzer is an unique drug discovery tool that screens and quantifies the impact of novel drugs on cell metabolism. The real-time measurement of the change in metabolism enables it to determine what becomes of cell viability, well-being, and metabolism as a result of drugs. In addition, LiCellMo can be used for optimizing the various R&D protocols (e.g. R&D of cell therapies and biologic drugs), or used as a quality control tool to track cell statuses. LiCellMo can accelerate and optimize drug development process, in which only viable candidate drugs with the highest prospects for the future proceed to clinical trials.
Advantages of Using the Live Cell Metabolic Analyzer
Data Reproducibility and Accuracy
Using the Live Cell Metabolic Analyzer, researchers can acquire data that is accurate and reproducible. This is important so that during R&D and pre-clinical studies, there is a guarantee that information collected is valid. Time taken to carry out research considerably decreases.
With the Live Cell Metabolic Analyzer, researchers can now make quicker decisions over current experiment results because the device provides continuous and real-time feedback of metabolism. Not only does it accelerate research, but it also saves time and human resources in conducting useless experiments.
Real-Time Insights for Dynamic Cellular Behavior
Compared to more traditional cell processing or fixation procedures, the Live Cell Metabolic Analyzer preserves the dynamic character of the living cell and gives insight into how it responds to treatments, stress, or other experimental conditions. This allows researchers and clinicians to make informed decisions in advance, based on real-time information.