Product under developmentCell Expansion System

Cell state visualization through continuous changes in glucose and lactate concentrations Optimal culture conditions maintained through automatic culture control,contributing to enhanced cell quality

Ensuring cell quality and stability is vitally important in the manufacture of cell and gene therapy products such as cells for CAR-T therapy.
However, manufacturing with cells as raw materials can be affected by factors such as variations in the manufacturing process and in the characteristics of the cells, which makes product quality unstable. Understanding the state of cells throughout the manufacturing process is thus an essential factor for enhancing the quality of cell and gene therapy products.

PHCbi offers an innovative solution—the LiCellGrow Cell Expansion System. Our unique In-Line Sensor monitors cell culture in real time, allowing automatic control of the culture to match the metabolic state of the cells while maintaining sterile conditions.
This enables the state of the cells to be quantified, greatly improving the efficiency and stability of the manufacturing process.

Continuous measurement by the In-Line Sensor allows visualization of changes in glucose and lactate concentrations Automatic culture medium replacement ensures an optimal culture environment at all times

Glycolysis is one of the major energy metabolism pathways in cells. Since this process takes in glucose and produces lactate, changes in these substances are considered to be a useful indicator of the cellular state. The LiCellGrow Cell Expansion System not only conventionally detects pH/DO in the medium, but also measures the concentrations of glucose and lactate in real time. It is also capable of automatically replacing the culture medium based on the measured glucose and lactate values.
The dedicated Culture Bag is fitted with the PHCbi In-Line Sensor, which provides real-time measurements of glucose and lactate as continuous line data. The measured values are converted to concentrations to visualize cell status, and this information is used for automatically replenishing the medium and draining waste.
This ensures that glucose and lactate concentrations in the medium are controlled and that cells are always cultured in their most suitable environment.

External view and dimensions

(unit:mm)

• Specifications may change at the time of marketing because the product is under development.
• Actual colors may vary slightly from the printed images.
• Product specifications, ratings, and design may be subject to change without notice due to improvements, etc.

Main product specifications

Product overview

Product overview	Name	Cell Expansion System (with glucose/lactate In-Line monitoring/control function)
	Monitoring parameters	• Glucose, lactate (simultaneous measurement of both parameters) • pH/DO (simultaneous measurement of both parameters) ※ pH/DO measurement is available as an option
	Main device components	Controller, Processor
	Main consumables (single use)	Culture bag, Sensor holder, Reservoir waste bag
	Culture Bag capacity	• 500 mL (minimum culture volume 100 mL) • 1000 mL (minimum culture volume 300 mL)
	Reservoir waste bag capacity	3000 mL
	Monitoring time	Maximum 10 days
	Measurement ranges	Glucose: 1–27 mM Lactate: 1.5–15 mM pH: 6.0 ~ 8.0 DO: 0 ~ 250% a.s. (a.s. = air saturation)

Controller

Controller	External dimensions	Width 371 mm × Depth 200 mm × Height 295 mm
	Screen	15.6-inch-wide touch panel display
	Weight	2.5 kg (excluding accessories)

Processor

Processor	External dimensions	Width 410 mm × Depth 410 mm × Height 400 mm
	Installation	Inside CO₂ incubator
	Weight	14kg ※1

※1 Please use the dedicated reinforced tray when installing the processor in a PHCbi CO2 incubator

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