PHCbi
PHC Corporation, Biomedical Division Live Cell Metabolic Analyzer Application Note

Application Note

Application Note

In-Line monitoring of culture medium using Live cell metabolic analyzer

In this study, we used a Live cell metabolic analyzer to continuously measure glucose and lactate levels while culturing Jurkat cells, in order to investigate the accuracy and stability of PHC’s proprietary In-Line sensor.

Download

Visualization of metabolic profile of immune cells using metabolic inhibitors

This study used THP-1 cells from an acute monocytic leukemia cell line (obtained from JCRB Cell Bank: JCRB0112.1), and NB-4 cells from an acute promyelocytic leukemia cell line (obtained from CLS - Cell Lines Service GmbH: 300299).
In this study, we attempted to visualize cell metabolic profiles by continuously measuring changes in cell metabolism using a glycolysis inhibitor and a mitochondrial metabolism inhibitor.

Download

Metabolic rate monitoring of undifferentiated iPS cells and expression analysis of differentiation markers

Since the state of undifferentiated iPSCs is a major factor in the quality and efciency of subsequent processes such as freezing stock and differentiation induction, it is necessary to understand the constantly changing state of the cells to carry out culture operations at the best possible time. PHCbi developed the Live cell metabolic analyzer, which can continuously measure glucose and lactate concentrations in the culture medium in common, commercially-available, 24-well plates through in-line monitoring. This allows changes in cell state over time to be quantified.

Download