PHCbi
PHC Corporation, Biomedical Division Live Cell Metabolic Analyzer

Live Cell Metabolic Analyzer

Continuous, sampling-free measurement of glucose and lactate in culture medium.
Visualize real-time changes in cell metabolism.

Cells are constantly growing and differentiating, and these processes are known to be closely linked to metabolism.
In the field of cancer immunology, stem cell research, and the development of manufacturing processes for cell-based formulations,
understanding the metabolic state of cells is a factor of ever-growing importance in the analysis of cell activation and disease.
Continuous analysis of cell metabolism offers the ability to visualize the state of cells in real time, creating opportunities for unprecedented new discoveries in cell metabolism.
The PHCbi Live cell metabolic analyzer will open new doors to those discoveries.

This product is for research use only.
Do not use for medical purposes.
  • Cancer
    research
  • Immunology
    research
  • Stem cell
    research
  • Metabolism
    Research
  • Process
    Development

In-Line sensors: the key to continuous measurements of glucose and lactate

The glycolytic pathway is one of the main components of cellular energy metabolism. During glycolysis, glucose is taken up into cells and lactate is produced. Conventional analysis of cell metabolism typically involves estimating glucose and lactate concentrations from data points obtained from periodic sampling. With its unique high-precision In-Line sensors, the PHCbi Live cell metabolic analyzer offers these unique advantages:

  • real-time monitoring of glucose and lactate concentrations
  • continuous measurements
  • no sampling of the culture medium and the same cells can also be used for separate evaluation after measurement

Measure cells in their usual culture environment

The simple design of the PHCbi Live cell metabolic analyzer makes it suitable for any laboratory space. Plus, there is no need for specialized cell culture equipment. Common commercial products (culture medium, 24-well plates, calibration liquid, additive reagents) can be used for cell culture. The Sensor module and Plate adaptor can be attached to a standard 24-well plate. Then, once the plate is placed in the Detector pre-installed within the CO2 incubator, real-time measurements can be checked easily using the touch-panel controller. Optional plate adapters for five different commercial 24-well plates are available.

Evaluate glycolytic changes directly

Changes in the glycolytic pathway can be evaluated directly by measuring the culture medium concentrations of glucose taken up by cells and lactate produced. With the PHCbi Live cell metabolic analyzer, the state of cell metabolism can be visualized as the rate of metabolic change using the consumption rate and production rate based on concentration values. Monitoring the efficiency of conversion from glucose to lactate makes it possible to evaluate not only glycolysis, but also changes in the balance with other cellular metabolic processes, such as oxidative phosphorylation.

References（Results：）

  • Yamamoto et al. Suppressive effect of the anesthetic propofol on the T cell function and T cell-dependent immune responses. Sci Rep 14:19337 (2024).
    PubMed: 39164311
  • Miki et al. Live-cell metabolic analyzer protocol for measuring glucose and lactate metabolic changes in human cells. STAR Protoc 6:103518
    PubMed: 39799573

Specifications

External view and dimensions (mm)

Cell Metabolism Analysis

Specifications

Product summary Items monitored Glucose, lactate (simultaneous continuous measurement of both items)
Main device components Controller, Detector, Plate adapter (optional product)
Main consumables Sensor module (single use)
Compatible plates 24-well plate *Compatible with 5 commercial products
Monitoring duration Maximum 10 days
Measurement range Glucose: up to 27 mM (up to 4.9 g/L)
Lactate: up to 15 mM (up to 1.4 g/L)
Detector External dimensions Width 162 mm × Depth 280 mm × Height 118 mm
Weight 4.7 kg
Installation Inside CO2 incubator
Recommended number of units to be installed in a CO2 incubator*
MCO-50 series MCO-170 series, MCO-171AICUVD MCO-230 series
Max. 1 unit Max. 4 units 92 units x 2 tiers) Max. 4 units 92 units x 2 tiers)
Controller External dimensions Width 371 mm × Depth 200 mm × Height 295 mm
Weight 2.5 kg (excluding accessories)
Screen 15.6-inch-wide touch panel display
Extendibility Wired connectivity for up to 4 detectors

*Specifications, ratings and design are subject to change without notice.